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Description
Buffers
We have over 50 years of experience in providing biological buffers which have been used as components in biopharmaceuticals, cell culture media, diagnostic reagents and life science research. Our biological buffers are scalable, regardless of need or requirement, are manufactured under stringent quality systems and are available in custom formats.
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent. Because of it’s ability to maintain physiological pH Hepes Buffer is widely used in cell cuture. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. According to it’s application, Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. |
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